primary rabbit polyclonal antibody il-6  (Thermo Fisher)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Panax notoginseng Alleviates Sepsis-Induced Acute Kidney Injury by Reducing Inflammation in Rats

doi: 10.1155/2022/9742169

Figure Lengend Snippet: PNP markedly reduced the secretion of peripheral blood cytokines induced by CLP in vivo . IL-18, IL-1 β , TNF- α , and IL-6 levels in kidney tissues were determined by ELISA. The results shown are representative of at least three independent experiments. Each value represents the mean ± SD.

Article Snippet: After that, TMA sections were incubated with rabbit antihuman primary polyclonal antibodies against IL-1 β , TNF- α , and IL-6 (Cell Signaling Technology, CST) overnight at 4°C and then incubated with a biotin-labeled secondary antibody (Invitrogen, Carlsbad, CA) at room temperature for 15 min, followed by incubation with HRP-conjugated streptavidin (Invitrogen, Carlsbad, CA) at room temperature for 15 min. Then, the color development was performed with a DAB Substrate Kit (Dako, Glostrup, Denmark).

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Panax notoginseng Alleviates Sepsis-Induced Acute Kidney Injury by Reducing Inflammation in Rats

doi: 10.1155/2022/9742169

Figure Lengend Snippet: PNP attenuated the expression of cytokines in the kidney tissues induced by CLP in vivo . The expression of IL-1 β , TNF- α , and IL-6 levels in kidney tissues was determined by immunohistochemical staining. The results shown are representative of at least three independent experiments. Scale bar: 10 μ m.

Article Snippet: After that, TMA sections were incubated with rabbit antihuman primary polyclonal antibodies against IL-1 β , TNF- α , and IL-6 (Cell Signaling Technology, CST) overnight at 4°C and then incubated with a biotin-labeled secondary antibody (Invitrogen, Carlsbad, CA) at room temperature for 15 min, followed by incubation with HRP-conjugated streptavidin (Invitrogen, Carlsbad, CA) at room temperature for 15 min. Then, the color development was performed with a DAB Substrate Kit (Dako, Glostrup, Denmark).

Techniques: Expressing, In Vivo, Immunohistochemical staining, Staining